不同提取方式对北板蓝根多糖含量和抗氧化活性的影响

王玉婷; 彭媛媛; 刘霞; 陈雪琴; 赵琳静

doi:10.12299/jsues.22-0054

不同提取方式对北板蓝根多糖含量和抗氧化活性的影响

doi: 10.12299/jsues.22-0054

上海工程技术大学化学化工学院, 上海 201620

基金项目: 国家自然科学基金项目资助（31701032）；上海工程技术大学研究生创新项目资助（0234-E3-0903-19-01359）

详细信息

作者简介:
王玉婷（1994−），女，在读硕士，研究方向为天然药物. E-mail：194588117@qq.com

通讯作者:
赵琳静（1979−），女，副教授，博士，研究方向为天然药物活性成分. E-mail：ljzhao@sues.edu.cn

中图分类号: R284.2
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出版历程
- 收稿日期: 2022-03-13
- 刊出日期: 2023-06-20

Effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen

School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

摘要

摘要: 考察不同提取方式对北板蓝根多糖含量及抗氧化活性的影响. 采用常规回流提取法和超声波辅助回流法提取北板蓝根多糖，通过苯酚−硫酸分光光度法测定总多糖含量，采用二苯代苦味酰基自由基（2,2−diphenyl−1−picryhydrazyl, DPPH·）和2,2−联氮−二（3−乙基−苯并噻唑−6−磺酸）二铵盐自由基（ABTS⁺·）清除实验评价体外抗氧化作用. 结果表明，超声波辅助回流法提取的多糖得率为40.07%，显著高于常规回流提取法（13.42%）. 苯酚−硫酸法的精密度高，重复性、稳定性和加样回收率良好，可用于不同提取方法制备的北板蓝根总多糖含量研究. 超声波辅助回流法提取的多糖含量比常规回流提取法提高22.3%，对DPPH 自由基和ABTS⁺自由基的清除作用显著优于常规回流提取法. 研究结果可为超声波技术在北板蓝根多糖提取中的应用提供参考.
- 北板蓝根 /
- 多糖 /
- 超声波辅助提取 /
- 抗氧化活性 /
- ABTS⁺自由基 /
- DPPH自由基
Abstract: The effects of different extraction methods on polysaccharide content and antioxidant activity of northern Ban-Lan-Gen were investigated. The polysaccharide of northern Ban-Lan-Gen was extracted by conventional reflux extraction method and ultrasonic assisted reflux extraction method. The total polysaccharide content was determined by phenol-sulfuric acid spectrophotometry. DPPH free radical and ABTS⁺ free radical scavenging assays were used to evaluate their antioxidant activity in vitro. The results show that the yield of polysaccharide extracted by ultrasonic assisted reflux extraction method was 40.07%, which was significantly higher than that by conventional reflux extraction method (13.42%). The phenol-sulfuric acid method has high precision, repeatability, stability and good recovery, which can be used to study the polysaccharide content of northern Ban-Lan-Gen prepared by different extraction methods. The polysaccharide content of ultrasonic assisted reflux extraction method was 22.3% higher than that of conventional reflux extraction method, and the scavenging effects of DPPH· and ABTS⁺· was significantly better than that of conventional reflux extraction method. The results provide a reference for the application of ultrasonic technology in the extraction of polysaccharide of northern Ban-Lan-Gen.
- northern Ban-Lan-Gen /
- polysaccharide /
- ultrasonic assisted extraction /
- antioxidant activity /
- ABTS⁺ free radical (ABTS⁺·) /
- DPPH free radical (DPPH·)

HTML全文

图 1 不同提取方式制备的北板蓝根多糖对 DPPH·的清除率

Figure 1. DPPH· scavenging rate of polysaccharide obtained by different extraction methods

下载: 全尺寸图片幻灯片

图 2 不同提取方式制备的北板蓝根多糖对 ABTS⁺自由基的清除率

Figure 2. ABTS⁺ free radical scavenging rate of polysaccharide obtained by different extraction methods

下载: 全尺寸图片幻灯片

表 1 加样回收率实验结果

Table 1. Results of recovery rate

样品中多糖质量/μg	加入对照品质量/μg	加样回收率/%	平均加样回收率/%	RSD/%
18.67	15	100.607
18.59	20	99.318	99.81	0.701
18.30	25	99.492

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表 2 北板蓝根多糖含量测定

Table 2. Determination of polysaccharide content obtained from northern Ban-Lan-Gen

提取方式	序号	浓度/(μg•mL⁻¹)	多糖含量/%	(平均值 ± 标准差)/%
常规回流法	1	18.50	4.63	4.63 ± 0.32
	2	18.59	4.65
	3	18.48	4.62
超声波辅助回流法	1	24.07	6.02	5.96 ± 1.11
	2	23.56	5.89
	3	23.91	5.98

下载: 导出CSV

参考文献(8)

[1]	DU Z J, LIU H, ZHANG Z L, et al. Antioxidant and anti-inflammatory activities of radix isatidis polysaccharide in murine alveolar macrophages[J] . International Journal of Biological Macromolecules,2013,58:329 − 335. doi: 10.1016/j.ijbiomac.2013.04.037
[2]	WANG T B, WANG X W, ZHUO Y, et al. Antiviral activity of a polysaccharide from Radix Isatidis (Isatis indigotica Fortune) against hepatitis B virus (HBV) in vitro via activation of JAK/STAT signal pathway[J] . Journal of Ethnopharmacology,2020,257:112782. doi: 10.1016/j.jep.2020.112782
[3]	LI J P, YUAN Y, ZHANG W Y, et al. Effect of Radix Isatidis polysaccharide on alleviating insulin resistance in type 2 diabetes mellitus cells and rats[J] . Journal of Pharmacy & Pharmacology,2019,71(2):220 − 229. doi: 10.1111/jphp.13023
[4]	JI H Y, YU J, JIAO J S, et al. Ultrasonic-assisted extraction of Codonopsis pilosula glucofructan: Optimization, structure, and immunoregulatory activity[J] . Nutrients,2022,14(5):927. doi: 10.3390/nu14050927
[5]	WU L, GAO Y, REN W C, et al. Rapid determination and origin identification of total polysaccharides contents in Schisandra chinensis by near-infrared spectroscopy[J] . Spectrochim Ica Acta Part A: Molecular and Biomolecular Spectroscopy,2022,264:120327. doi: 10.1016/j.saa.2021.120327
[6]	THETSRIMUANG C, KHAMMUANG S, CHIABLAEM K, et al. Antioxidant properties and cytotoxicity of crude polysaccharides from Lentinus polychrous Lév[J] . Food Chemistry,2011,128(3):634 − 639.
[7]	马黄璜, 郭晓芳, 张增弟, 等. 不同提取方式对金线莲多糖含量及DPPH自由基清除活性的影响[J] . 海峡药学,2020,32(1):34 − 36. doi: 10.3969/j.issn.1006-3765.2020.01.010
[8]	曾小燕, 李鸿霞, 梁纪强, 等. 中药多糖体外抗氧化作用研究进展[J] . 亚太传统医药,2020,16(7):181 − 188.