Volume 35 Issue 1
Sep.  2022
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HAO Fangjia, ZHANG Ning, YANG Juan, ZHAO Linjing. GC-MS analysis, antimicrobial and antioxidant activities of volatile constituents from Isatidis Radix (Banlangen)[J]. Journal of Shanghai University of Engineering Science, 2022, 36(1): 41-45, 104. doi: 10.12299/jsues.21-0205
Citation: HAO Fangjia, ZHANG Ning, YANG Juan, ZHAO Linjing. GC-MS analysis, antimicrobial and antioxidant activities of volatile constituents from Isatidis Radix (Banlangen)[J]. Journal of Shanghai University of Engineering Science, 2022, 36(1): 41-45, 104. doi: 10.12299/jsues.21-0205

GC-MS analysis, antimicrobial and antioxidant activities of volatile constituents from Isatidis Radix (Banlangen)

doi: 10.12299/jsues.21-0205
  • Received Date: 2021-09-28
  • Publish Date: 2022-09-26
  • The aim of this study was to determine the chemical constituents of volatile constituents from Isatidis Radix, and evaluate the antimicrobial and antioxidant activities using in vitro assays, including disk diffusion test, prussian blue test, ferric reducing antioxidant power and 1, 1-diphenyl-2-trinitrophenylhydrazine free radical (DPPH·) scavenging activity. Results showed that the volatile constituents of Isatidis Radix had low content (0.038%) and comprised of isothiocyanates, aromatic hydrocarbons, carboxylic acids, etc., which were identified by NIST Library. The volatile constituents of Isatidis Radix had antibacterial effects on Escherichia coli and Staphylococcus aureus, and the average antibacterial zone diameters were 8.1 mm and 6.5 mm, respectively. Also, it had good reducing ability, and obvious scavenging effect on DPPH·, with IC50 value of 7.169 mg/mL.

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