Optimization of preparation process of clove volatile oil inclusion complex by Box-Behnken response surface methodology

MA Ying; LIU Yurou; LIU Xiaohui

doi:10.12299/jsues.24-0085

Volume 39 Issue 2

Jun. 2025

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Article Navigation > Journal of Shanghai University of Engineering Science > 2025 > 39(2): 174-180

MA Ying, LIU Yurou, LIU Xiaohui. Optimization of preparation process of clove volatile oil inclusion complex by Box-Behnken response surface methodology[J]. Journal of Shanghai University of Engineering Science, 2025, 39(2): 174-180. doi: 10.12299/jsues.24-0085

Citation:

MA Ying, LIU Yurou, LIU Xiaohui. Optimization of preparation process of clove volatile oil inclusion complex by Box-Behnken response surface methodology[J]. Journal of Shanghai University of Engineering Science, 2025, 39(2): 174-180. doi: 10.12299/jsues.24-0085

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Optimization of preparation process of clove volatile oil inclusion complex by Box-Behnken response surface methodology

doi: 10.12299/jsues.24-0085

School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China

Received Date: 2023-03-23
Available Online: 2025-09-30
Publish Date: 2025-06-30

Abstract

Abstract

To prepare the hydroxypropyl-β-cyclodextrin inclusion complex from clove essential oil using saturated aqueous solution method. Based on the single factor experiments, the ratio of hydroxypropyl-β-cyclodextrin to volatile oil, encapsulation temperature, and encapsulation time were used as influencing factors, and the comprehensive score was used as the response value. The encapsulation process was optimized by Box-Behnken response surface methodology. The optimal condition was obtained as the ratio of hydroxypropyl-β-cyclodextrin to volatile oil was 14.5∶1, with an inclusion temperature of 59 ℃ and an inclusion time of 2.4 h. The comprehensive score of the three validation experiments is 92.21%±0.50%, which is close to the predicted value, indicating that the prediction of the model is accurate and reliable. The inclusion complex was observed under microscope, analyzed by thin layer chromatography (TLC), and identified by infrared spectroscopy, proving the formation of a stable inclusion complex. These results can provide data support for further development and utilization of clove volatile oil.
- clove volatile oil,
- hydroxypropyl-β-cyclodextrin,
- process optimization,
- Box-Behnken response surface methodology

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References(18)

References

[1]	XUE Q, XIANG Z D, WANG S G, et al. Recent advances in nutritional composition, phytochemistry, bioactive, and potential applications of Syzygium aromaticum L. (Myrtaceae)[J] . Frontiers in Nutrition, 2022, 9: 1002147. doi: 10.3389/fnut.2022.1002147
[2]	ZHANG P, ZHANG E L, XIAO M, et al. Enhanced chemical and biological activities of a newly biosynthesized eugenol glycoconjugate, eugenol α-D-glucopyranoside[J] . Applied Microbiology and Biotechnology, 2013, 97(3): 1043 − 1050. doi: 10.1007/s00253-012-4351-2
[3]	HAN X S, PARKER T L. Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts[J] . Pharmaceutical Biology, 2017, 55(1): 1619 − 1622. doi: 10.1080/13880209.2017.1314513
[4]	ZHANG L F, GU B, WANG Y. Clove essential oil confers antioxidant activity and lifespan extension in C. elegans via the DAF-16/FOXO transcription factor[J] . Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2021, 242: 108938.
[5]	SINGH J, BAGHOTIA A, GOEL S P. Eugenia caryophyllata thunberg (Family Myrtaceae): a review[J] . International Journal of Research in Pharmaceutical and Biomedical Sciences, 2012, 3(4): 1469 − 1475.
[6]	LI Z H, LI K, TENG M L, et al. Functionality-related characteristics of hydroxypropyl-β-cyclodextrin for the complexation[J] . Journal of Molecular Liquids, 2022, 365: 120105. doi: 10.1016/j.molliq.2022.120105
[7]	GHARIB R, GREIGE-GERGES H, FOURMENTIN S, et al. Hydroxypropyl-β-cyclodextrin as a membrane protectant during freeze-drying of hydrogenated and non-hydrogenated liposomes and molecule-in-cyclodextrin-in- liposomes: application to trans-anethole[J] . Food Chemistry, 2018, 267: 67 − 74. doi: 10.1016/j.foodchem.2017.10.144
[8]	方浩标, 李春海, 张钟, 等. 响应面试验优化饱和水溶液法制备紫苏精油β-环糊精包合物工艺及其理化性质[J] . 绿色包装, 2019(1): 47 − 54.
[9]	韩林, 张艳侠. 丁香挥发油β-环糊精包合物的制备研究[J] . 西北药学杂志, 2011, 26(6): 447 − 449. doi: 10.3969/j.issn.1004-2407.2011.06.024
[10]	李双双, 李希, 邓谦, 等. 椒香温中止痛方挥发油β-环糊精包合工艺优化及其包合物表征[J] . 中成药, 2020, 42(12): 3128 − 3134. doi: 10.3969/j.issn.1001-1528.2020.12.003
[11]	王琪, 胡佳亮, 张金良, 等. 薄荷-荆芥穗挥发油羟丙基-β-环糊精包合物的制备[J] . 中成药, 2020, 42(12): 3122 − 3128. doi: 10.3969/j.issn.1001-1528.2020.12.002
[12]	代良敏, 代良萍, 陈永钧, 等. 基于Box-Behnken实验优化地榆皂苷元固体脂质纳米粒的制备[J] . 时珍国医国药, 2023, 34(9): 2117 − 2120. doi: 10.3969/j.issn.1008-0805.2023.09.17
[13]	ABD-EL-AZIZ N M, HIFNAWY M S, EL-ASHMAWY A A, et al. Application of Box-Behnken design for optimization of phenolics extraction from Leontodon hispidulus in relation to its antioxidant, anti-inflammatory and cytotoxic activities[J] . Scientific Reports, 2022, 12(1): 8829. doi: 10.1038/s41598-022-12642-2
[14]	KUSHWAHA P, SHUKLA B, DWIVEDI J, et al. Validated high-performance thin-layer chromatographic analysis of curcumin in the methanolic fraction of Curcuma longa L. rhizomes[J] . Future Journal of Pharmaceutical Sciences, 2021, 7(1): 178. doi: 10.1186/s43094-021-00330-3
[15]	TORIMOTO T, YAMAGUCHI N, MAEDA Y, et al. Development of plasmonic thin-layer chromatography for size-selective and optical-property-dependent separation of quantum dots[J] . NPG Asia Materials, 2022, 14(1): 64. doi: 10.1038/s41427-022-00414-3
[16]	YUAN C, JIN Z Y, XU X M. Inclusion complex of astaxanthin with hydroxypropyl-β-cyclodextrin: UV, FTIR, ¹H NMR and molecular modeling studies[J] . Carbohydrate Polymers, 2012, 89(2): 492 − 496. doi: 10.1016/j.carbpol.2012.03.033
[17]	YUAN C, LIU B G, LIU H. Characterization of hydroxypropyl-β-cyclodextrins with different substitution patterns via FTIR, GC-MS, and TG-DTA[J] . Carbohydrate Polymers, 2015, 118: 36 − 40. doi: 10.1016/j.carbpol.2014.10.070
[18]	HAO X C, SUN X D, ZHU H Z, et al. Hydroxypropyl-β-cyclodextrin-complexed resveratrol enhanced antitumor activity in a cervical cancer model: in vivo analysis[J] . Frontiers in Pharmacology, 2021, 12: 573909. doi: 10.3389/fphar.2021.573909