Preparation and research on antibacterial activity of inclusion compound of basil volatile oil
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摘要: 水蒸气蒸馏法提取挥发油后，用羟丙基-β-环糊精制备包合物，用紫外分光光度计测定其含油量. 通过显微镜法、薄层色谱（TLC）法、红外光谱（IR） 法对其结构进行表征. 结果显示，包合物收率为52.23%，包合率为2.82%，含油率为1.02%. 采用纸片法和二倍稀释法测定包合物对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑菌圈和最小抑菌浓度（MIC），结果显示抑菌圈直径分别为1.74、0.82和1.12 cm，最小抑菌浓度分别为3.79、8.58和3.79 mg/mL.Abstract: After the volatile oil was extracted by steam distillation, hydroxypropyl-β-cyclodextrin was used to refine the inclusion compound, and the oil content was determined by ultraviolet spectrophotometer. Characterization was accomplished by microscope, thin-layer chromatography (TLC) and infrared spectroscopy (IR). The results show that the yield of inclusion compound is 52.23%, the inclusion rate is 2.82%, and the oil content is 1.02%. The inhibition zone and minimum inhibitory concentration (MIC) of the inclusion complex against Escherichia coli, Staphylococcus aureus and Candida albicans were determined by disk method and two-fold dilution method. The results show that the inhibition zone are 1.74, 0.82 and 1.12 cm respectively, and the minimum inhibitory concentration are 3.79, 8.58 and 3.79 mg/mL respectively.
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图 2 薄层色谱图
A—羟丙基-β-环糊精；B—罗勒挥发油；C—包合物；D—包合物中提取的挥发油.
Figure 2. TLC chromatogram of samples
表 1 羟丙基-β-环糊精包合结果
Table 1. Inclusion results of Hydroxypropyl- β- Cyclodextrin
包合材料 含油量 包合率/% 包合物收率/% 含油率/% 羟丙基-β-环糊精 0.00678 2.82 53.23 1.02 
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表 2 包合物抑菌圈直径
Table 2. Inhibition zone of inclusion complex
cm 包合物质量浓度/
(mg·mL−1)抑菌圈直径/cm 大肠杆菌 金黄色葡萄球菌 白色念珠菌 944 1.74 0.82 1.12
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