Therapeutic effect of Bafukang suppository on Candida tropicalis infection
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摘要: 为验证保妇康栓体内外抗热带假丝酵母菌的药效，从临床收集热带念珠菌16株，采用微量肉汤稀释法测定保妇康栓对热带假丝酵母菌的最低抑菌浓度(MIC)，用结晶紫染色法测定对生物膜形成的影响，用扫描电镜(SEM)观察对菌体结构的影响。构建小鼠阴道热带假丝酵母菌体内感染模型，观察给予保妇康栓后阴道荷菌量的变化及阴道病理的改变。结果表明保妇康栓可抑制热带假丝酵母菌的增殖和生物膜的形成，破坏菌体细胞膜和细胞壁，并有效缓解小鼠阴道炎症，在体外对热带假丝酵母菌的生长具有显著抑制作用，在体内对热带假丝酵母菌感染致阴道炎有明显的改善作用。Abstract: To verify the efficacy of Baofukang suppository against Candida tropicalis in vivo and in vitro, a total of 16 clinical strains of Candida tropicalis were collected. The minimum inhibitory concentration (MIC) of Baofukang suppository against Candida tropicalis was determined using microbroth dilution method. The impact on biofilm formation was evaluated by crystal violet staining method, and the alternations in fungus structure was observed using scanning electron microscope (SEM). The vaginal Candida tropicalis infection model in mice was established, changes in vaginal fungus load and vaginal pathology after giving Baofukang suppository were monitored. The results show that Baofukang suppository can inhibit the proliferation and biofilm formation of Candida tropicalis, destroy the cell wall and membrane of Candida tropicalis, effectively relieve vaginal inflammation in mice. Baofukang suppository had obvious inhibitory effect on the growth of Candida tropicalis in vitro and ameliorative effect on vaginitis caused by Candida tropicalis infection in vivo.
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图 1 400倍倒置显微镜下保妇康栓对热带假丝酵母菌生物膜形成的影响
Figure 1. Inhibition of Baofukang suppository on biofilm formation of Candida tropicalis under 400 fold inverted microscope
图 5 各组小鼠阴道组织病理评分变化情况
Figure 5. Changes of pathological score of vaginal tissue of mice in each group
图 6 各组小鼠阴道组织病理学观察（HE， × 200）
Figure 6. Observation of vaginal histopathology in each group of mice
表 1 药敏实验结果
Table 1. Results of drug sensitivity test
菌株编号 克霉唑MIC值/(μg·mL−1) 保妇康栓MIC值/(mg·mL−1) 10705 0.50 0.64 10808 8.00 0.16 0121042 0.50 0.16 17040 0.50 0.64 09038 0.50 0.64 07703 4.00 0.16 10002 4.00 0.16 05058 0.03 0.32 19021 1.00 0.64 07073 1.00 0.32 18004 0.03 0.32 08003 0.06 0.64 0121056 0.25 0.32 ATCC750 0.50 0.16 0119043 4.00 0.16 09020 0.25 1.28 23601 0.25 2.56 
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